rabbit polyclonal anti tcp1  (Bethyl)

Journal: Cell

Article Title: Mechanism of orphan subunit recognition during assembly quality control

doi: 10.1016/j.cell.2023.06.016

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-TCP1 , Bethyl , Cat#A303-445A; RRID: AB_10948976.

Techniques: Recombinant, Transfection, Protease Inhibitor, Mutagenesis, In Vitro, Plasmid Preparation, Negative Control, Sequencing, Expressing, Software, Western Blot, Clear Native PAGE

Journal: bioRxiv

Article Title: Uncovering protein-protein interactions of the human sodium channel Nav1.7

doi: 10.1101/2023.04.18.537407

Figure Lengend Snippet: A . Schematic illustrating the procedures involved in the affinity purification, SEC, and MS/MS of TAP-tagged hNav1.7. B . TAP-tagged NaV1.7 was detected using western blotting with anti-FLAG antibody after single-step and tandem affinity purification (SS-AP and TAP). Ci . A representative size exclusion chromatography of the protein sample. Cii . The resolved fractions isolated from size exclusion chromatography visualized using Coomassie blue staining. D . The validation of selected Nav1.7 protein interactor candidates Kif11, CCT5, and TMED10, where Nav1.7 complexes were immunoprecipitated with anti-FLAG M2 magnetic beads and then detected with their specific antibodies using Western blotting.

Article Snippet: The membranes were blocked with 5% nonfat milk for 30 minutes before being treated with primary antibodies, including anti-FLAG (1:1000, Sigma, F1804), anti-Nav1.7 (1:500, Origene, TA329033), anti-CCT5 (1:1000, Origene, TA308298), and anti-TMED10 (1:1000, Origene, TA306375), overnight at 4°C.

Techniques: Affinity Purification, Tandem Mass Spectroscopy, Western Blot, Size-exclusion Chromatography, Isolation, Staining, Immunoprecipitation, Magnetic Beads

Journal: bioRxiv

Article Title: Uncovering protein-protein interactions of the human sodium channel Nav1.7

doi: 10.1101/2023.04.18.537407

Figure Lengend Snippet: A . Expression of CCT5 in HEK293 cells in response to the transfection of CCT5 siRNA. n = 4, P < 0.05. B . Expression of TMED10 in HEK293 cells in response to the transfection of CCT5 siRNA. n = 4, P < 0.05. C-E . Representative raw current traces of NaV1.7 from HEK293 cells stably expressing the TAP-tagged NaV1.7 in response to the activation pulse protocol shown. Each trace shows a different condition. F . IV plot of Nav1.7 current density in the absence and presence of CCT5 siRNA. Compared with NaV1.7 basal currents (n = 12), CCT5 siRNA transfection significantly reduced the sodium channel density (n = 10, P < 0.01). G . IV plot of Nav1.7 current density in the absence and presence of TMED10 siRNA. Compared with NaV1.7 basal currents (n = 10), CCT5 siRNA transfection significantly reduced the sodium channel density (n = 10, P < 0.05).

Article Snippet: The membranes were blocked with 5% nonfat milk for 30 minutes before being treated with primary antibodies, including anti-FLAG (1:1000, Sigma, F1804), anti-Nav1.7 (1:500, Origene, TA329033), anti-CCT5 (1:1000, Origene, TA308298), and anti-TMED10 (1:1000, Origene, TA306375), overnight at 4°C.

Techniques: Expressing, Transfection, Stable Transfection, Activation Assay

Journal: Frontiers in Molecular Biosciences

Article Title: Muscle Histopathological Abnormalities in a Patient With a CCT5 Mutation Predicted to Affect the Apical Domain of the Chaperonin Subunit

doi: 10.3389/fmolb.2022.887336

Figure Lengend Snippet: Primary antibodies used for immunohistochemistry (IH), and immunofluorescence and double immunofluorescence (IF).

Article Snippet: IF , CCT5 , Rabbit polyclonal , Origene , TA308298 , 1:50.

Techniques: Immunohistochemistry, Immunofluorescence